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NWFSC Aquaculture and Genetics Support
Ocean Associates
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Seattle, WA, USA
Posted 6+ months ago
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Ocean Associates Inc. (OAI) is seeking an applicant to provide Aquaculture and Genetics Support to the National Oceanic and Atmospheric Administration (NOAA), National Marine Fisheries Service (NMFS), Northwest Fisheries Science Center (NWFSC), Environmental and Fisheries Sciences (EFS) Division in Seattle, WA. OAI is a Virginia corporation established in 2003 that provides consulting and technical services to the U.S. government, non-governmental organizations, international organizations, and the private sector. We specialize in scientific program and project management, strategic planning, professional and technical services, and stakeholder engagement, supporting government contracts. We are seeking applicants for this position prospectively, which is dependent on award of contract.
Background
The NWFSC, Environmental Physiology Program is conducting research aimed at improving the environmental sustainability and economic feasibility of marine aquaculture. Methods for induction of reproductive sterility are broadly needed in marine aquaculture due to biosafety concerns associated with escape of farmed fish and potential genetic contamination of wild stocks. This project seeks to develop non-GMO methods for sterilization that can be applied on a large scale by the aquaculture industry to shellfish and finfish species. In addition, the development of an “analytical tool-kit” to assess physiological parameters in cultured shellfish that may be predictive of multi-stressor resistance (e.g., resistance to ocean acidification and elevated temperature) in the field is highly needed. Research under this project focuses on reproductive physiology in marine species and the development of predictive biomarkers of multi-stress resistance in cultured shellfish species.
Description
The project for which services are needed focuses on development of methods for reproductive sterilization of shellfish and finfish aquaculture species and the development and validation of physiological assays that can be used to predict multi-stress resistance in cultured shellfish. These are key research areas for the Environmental Physiology Program at the NWFSC that have been supported by the NOAA Office of Aquaculture and Ocean Acidification Program. Research activities shall include: 1) assisting with rearing and manipulation of experimental animals in controlled, captive conditions; 2) planning for and collecting tissue samples from experimental animals for downstream analysis; 3) organizing samples and conducting laboratory analyses using histology and microscopy, molecular biology techniques, and cellular/chemical bioassays; 4) organizing, analyzing, graphing and summarizing data obtained; 5) assisting with preparation of written reports and peer-reviewed publications, as well as slide decks for scientific presentations.
Tasks
1. Shellfish/fish culture and experimental manipulations
Conduct shellfish husbandry and manipulations of experimental fish and shellfish held in controlled conditions at the NWFSC Montlake Lab and/or Manchester Research Station. This may entail set up and maintenance of experimental systems for multi-stressor shellfish trials, daily care and feeding of experimental shellfish, experimental diet preparation and feeding fish, enumerating larvae or fish, testing water quality, cleaning tanks and net pens, and modifying tank systems and seawater lines as needed.
2. Tissue collections
Prepare supplies for tissue sampling events and assist staff scientists with tissue collections. Preparation for sampling events typically entails labeling tubes for collection of biological tissues, preparing field note sheets and packing supplies for sampling. Sampling itself typically entails collecting size data (length, weight, and tag number where applicable) and tissue samples from experimental animals using an RNase-free technique. Biological samples shall be properly stored for later analysis.
3. Laboratory analyses
Conduct laboratory analyses of various physiological factors in shellfish and finfish, including histology and microscopy, molecular biology techniques, immuno- and enzyme assays, and metabolic rate assays measured via respirometry. It is likely that optimization of established methodologies will be required for a particular species/life-stage experimental paradigm. Specific laboratory techniques that shall be used are: 1) paraffin histology (tissue processing, embedding, sectioning, staining) and microscopy to assess cellular and anatomical features of the gonad or other tissues; 2) molecular biology techniques such as DNA and RNA isolations and sequencing, gel electrophoresis, oligo primer design, standard and quantitative PCR, targeted gene cloning, gene knock-downs and in situ hybridizations; 3) cellular and chemical bioassays for shellfish and fish tissues, including enzyme activity assays and hormone bioassays; and 4) metabolic rate assays measured via respirometry and/or metabolic proxy, such as resazurin assay.
4. Data entry, organization, analysis and presentation
Enter and compile data and notes from all laboratory and field activities in Microsoft Excel spreadsheets. Conduct initial statistical analyses using standard statistical software (e.g., R or Prism) and generate preliminary graphs/tables and data summaries for review by the Project Leader. Hard copies of data sheets from field sampling shall be kept in a secure location, while all electronic data shall be stored on a NWFSC computer and backed up to external hard drives to be provided by the NWFSC. Assist with writing materials and methods and other sections for annual reports and peer-reviewed publications, and assist with developing slide decks for presentations at scientific meetings. Provide a monthly status progress report (via email), to include, but not necessarily limited to, the following: accomplishments, issues encountered, travel (including purpose, significant outcomes, action items), and recommendations, if applicable.
Start Date: Contingent upon award of contract.
Location: Northwest Fisheries Science Center Seattle, WA.
Travel: Some travel may be required.
Salary and Benefits: This is a full-time position with benefits. Salary, commensurate with experience, between $23 - $35 per hour.
Requirements
Applicants must have the following minimum requirements:
- Bachelor's degree, or higher, from an accredited college or university with a major directly related in a field of study as related to the requirements of specific task order, with emphasis in aquaculture, fisheries, molecular biology or oceanography, plus one (1) year of experience in related field or combination of such totaling five (5) years of combined education/experience. Master’s Degree may be substituted for experience.
- Knowledge and experience working in a research laboratory.
- Knowledge of husbandry and reproduction of shellfish and/or fish.
- Knowledge of and experience with molecular biology techniques, including deoxyribonucleic acid (DNA) isolations, gel electrophoresis, and polymerase-chain reaction (PCR) assays. Knowledge of cellular and biochemical assays such as enzyme activity and enzyme-linked immunosorbent assays.
- Experience with graphing and statistical software, such as Microsoft Excel and Prism.
- Experience developing data summaries and writing methods from research conducted.
- Ability to lift two, 5-gallon buckets full of water and carry them 30 yards.
- Valid driver's license to allow for driving Government-Owned Vehicles.
- Basic computer skills (MS Office, etc.).
- Excellent verbal and written communication skills.
- Ability to work effectively both individually and collaboratively in a team/group setting.
- Ability to receive constructive feedback and implement appropriate action.
Additional preferred qualifications include:
- Experience writing materials and methods sections for scientific reports and/or publications.
- Experience with ribonucleic acid (RNA) extractions and in situ hybridization.
- Experience culturing aquatic species, such as fish and/or shellfish.
If you are interested in being considered for this position, please APPLY THROUGH OUR ADP WEB PORTAL, AT THE LINK AT THE TOP RIGHT OF THIS PAGE.
Only qualified applicants that meet minimum experience or background requirements stated above need apply. When applying for this position you will be asked to upload your resume at the end of this online application.
Applicants should submit a resume that includes the following:
- Cover letter that briefly describes how you meet the required and preferred qualifications listed.
- Work history for past 10 years or since last full-time education.
- Education.
- Previous experience or training with similar requirements.
- Three professional references.
- Include your name in the document file name.
- Upload your resume in readable, not scanned, PDF or Word format (PDF is preferred).
In compliance with federal law, all persons hired will be required to verify identity and eligibility to work in the United States, complete the required employment eligibility verification document form upon hire, and successfully complete a federal government background check. Ocean Associates Inc. is an Equal Opportunity Employer and does not unlawfully discriminate on the basis of any status or condition protected by applicable federal or state law.
OAI is a government contractor and is required by law to invite applicants to self-identify their race, gender, national origin, disability status and veteran status. OAI is required to use the information it collects as part of its future affirmative action efforts in hiring. The information is collected anonymously by computer and is not connected to your application. You will see a place to check certain boxes in OAI's online application. Even though the information is anonymous, self-identification is voluntary. You can always check the box that says you decline to self-identify.
Note: If you are not selected, your materials may be retained for present or future opportunities. We may use your resume in bidding on a project and if we are awarded the work, we will contact you before seeking further applicants. If you do not wish us to retain or use your resume to seek work for you, please let us know in the "Notes" section on the second page of the online application.
This job is no longer accepting applications
See open jobs at Ocean Associates.See open jobs similar to "NWFSC Aquaculture and Genetics Support" Schmidt Marine.